The Palace Museum in Beijing, also known as the Forbidden City, capped the amount of visitors to 80,000 per day on Saturday.
The museum also now requires all travel agencies book tickets online and demand the use of ID when purchasing tickets.
There were around 50,000 visitors at the museum on Saturday, which marked the country's 10th Cultural Heritage Day.
The new measures are part of its efforts to better protect the palace, which became a UNESCO World Heritage Site in 1987.
Meanwhile, the museum will try to improve its service to tourists, said an official of the museum.
Located in the heart of Beijing, the Forbidden City was home to China's emperors and was the highest center of power from 1420 to 1911. It attracts more than 15 million visitors annually.