John Mayer and Jennifer Aniston Despite wowing Hollywood with a high-profile Oscar date, Jennifer Aniston and John Mayer have ended their relationship once again, according to a source.

The couple was seen arm-in-arm at the Oscars and then Jennifer Aniston headed to Europe to promote Marley & Me and John Mayer stayed in Los Angeles to work on his music. After Aniston returned, they were a couple no more, the sources said.

"Aniston and Mayer had some disagreements and decided to not continue to see each other," according to the source "Jen is moving on with her life like she always does. She seems happy."

This is not the first time Aniston, 40, and Mayer, 31, have split. In August 2008, they called it quits. At the time Mayer held an impromptu press conference outside his gym in New York, explaining to the media: "I ended a relationship to be alone because I don't want to waste somebody's time if something's not right."

The romance between fourty-year-old Aniston and singer-songwriter Mayer, 31 began after meeting at the Academy Awards in 2008, and the two starlets quickly became a "hot couple" in the world of celebrity journalism.

(CRI March 14, 2009)